Purification and Determination of Cholest-4-en-3-one From Syngnathus, one of the Precious Chinese Medicine

Syngnathus is a kind of precious Chinese traditional medicine,which has many effects in kidney potency.Modern biochemistry and pharmacology studies have shown that its efficacies are closely related to the sterol composition in Syngnathus.We separated,purified and structurally determined a kind of sterol,cholest-4-en-3-one via HPLC and MS.The quantitative determination was experimentized,as a result,that steroid has a percentage of 0.177\% in Syngnathus,cholest-4-en-3-one is in the proportion of 9.39\% in the total sterol.Our research has laid a foundation for effective component standardization of traditional Chinese medicine,Syngnathus.