Laser Tweezers Raman Spectroscopy Analysis of Antarctica Bacterium Shewanella sp.NJ49

In the present study,laser tweezers Raman spectroscopy (LTRS) was employed to investigate single cell growth and dynamic changes of life materials inside and outside cell of psychrophilic petroleum hydrocarbon-degrading bacterium Shewanella sp.NJ49 isolated from Antarctica.This bacterium can degraded diesel,n-hexadecane and other petroleum hydrocarbons with high efficiency at low temperature;long straight chain hydrocarbons can be decomposed into short straight chain hydrocarbons;The result of Raman spectrum showed that the main signals detected were proteins and carbohydrate signals,and less was lipid signal.The Raman intensity at 1514\mathrm~cm^-1 represented \beta-carotene which involved in the regulation of membrane fluidity in Antarctic bacteria for adaptation in Antactica environment of extreme low temperature.