珊瑚白化反应:来自醇类生物标志物的启示

Coral Bleaching Response: Implications From Alcohol Biomarkers

  • 摘要: 珊瑚白化是珊瑚礁生态系统面临的严峻威胁之一。随着温度的快速升高和人类活动影响的持续加剧,珊瑚白化的生理生态反应和弹性恢复能力受到越来越多的关注。本文将小粒菊花珊瑚Goniastrea minuta和丛生盔形珊瑚Galaxea fascicularis作为研究对象,对比分析健康珊瑚和白化珊瑚之间的醇类生物标志物(正构醇和类固醇)含量、组成和稳定碳同位素丰度(δ13C)特征,以揭示珊瑚白化前后有机质来源和通量的变化过程,并阐明白化珊瑚的恢复方式和能力。研究结果发现,在健康的G. minutaG. fascicularis中,偶碳数(C16-18)正构醇占比最高(分别为79.7%和79.8%),而δ13C值偏正(分别平均为−17.6‰和−18.9‰);类固醇分子和δ13C组成基本一致。当珊瑚白化后,C16-18正构醇含量均显著减少,而与之相反C22-28正构醇呈升高趋势;类固醇含量减少,但幅度不一,其组成变化显著,类固醇δ13C组成呈现负偏的趋势。研究表明,白化的G. minutaG. fascicularis主要以异养恢复为主,但整体恢复速率较慢或效率较低,可能与珊瑚礁区寡营养环境有关,缺乏充足的食物来源。此外,在珊瑚中检测到C31-35酮类系列物,推断为人类活动(生物质燃烧)产物,表明人为污染叠加气候变化可能加剧珊瑚白化。由此可见,将正构醇和类固醇作为珊瑚中的脂类生物标志物进行研究,可以揭示珊瑚中醇类化合物的主要来源以及白化珊瑚的恢复方式,为珊瑚科学研究作出重要贡献。

     

    Abstract: Coral bleaching is one of the most serious threats to coral reef ecosystem. With the rapid rise of temperature and the continuous intensification of human activities, more and more attention has been paid to the physiological and ecological response and resilience of coral bleaching. In this study, we studied Goniastrea minuta and Galaxea fascicularis, and analyzed the composition, content and δ13C characteristics of alcohol biomarkers (n-alkanols and steroid) between healthy and bleaching corals. The changes of organic matter sources and flux before and after coral bleaching were revealed, and the recovery mode and ability of coral bleaching were elucidated. The results showed that in healthy G. minuta and G. fascicularis, the proportion of C16-18 n-alkanols was the highest (79.7% and 79.8%, respectively), and the δ13C value was positive (mean −17.6‰ and −18.9‰). The steroid molecules and δ13C composition are basically the same. After bleaching, the content and proportion of n-alkanols in C16-18 decreased significantly, while that in C22-28 increased in the opposite trend. The steroid content decreased, but the amplitude was different, and the composition changed significantly, but the steroid δ13C showed a negative composition trend. Studies have shown that G. minuta and G. fascicularis are mainly heterotrophic, but the overall recovery rate is slower or less efficient, which may be related to the oligotrophic environment in the reef area and the lack of adequate food sources. In addition, the C31-35 ketone compounds was detected in corals, which is inferred to be the product of human activities (biomass burning), man-made pollution combined with climate change, which can exacerbate coral bleaching and inhibit coral recovery. Therefore, the study of n-alkanols and steroid as lipid biomarkers in corals can reveal the main sources of alcohol compounds in corals and the recovery mode of bleached corals, which can make important contributions to coral scientific research.

     

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