南极真菌Geomyces sp.产色素条件和色素提取的研究

Study on the Condition of Production and Extraction of Pigment From an Antarctic Fungus Geomyces sp.

  • 摘要: 从南极土样中分离得到一株能大量分泌紫红色色素的地丝霉属(Geomyces)的真菌。通过对培养基和温度的选择,确定了该菌株产色素的基础培养基,发现较低的温度有利于色素的积累。另外,对7种树脂吸附量、解吸率和提取效率进行了比较,选择了HPD-722型树脂吸附色素,洗脱剂为70%乙醇。经过大孔树脂吸附法提取,色素色价从12.68提高到144.05,增高10.4倍,效果非常明显。碳源的加入对电池阳极表面菌群的种类组成有较大的扰动,导致细菌种类多样性的降低,其中华丽杆菌(Chryseobacterium sp.)为易感种类。

     

    Abstract: A Geomyces species was isolated from Antarctica soil,which produces plenty of red pigment.With screening test of culture medium and temperature,the basic medium was determined in which the pigment was produced,and it was discovered that low temperature benefited the accumulation of pigment in the fungus.In addition,compared the adsorption capacities and desorption rates of several macroporous absorption resin,HPD-722 resin and 70\% ethanol were determined for pigment absorption and eluting reagent.After extraction and absorption by macroporous resin,the Color Value Units E_1cm^1\% increased from 12.68 to 144.05,a 10.4 times increase was recorded.

     

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