4株海洋沉积物来源青霉真菌的生理学特性研究

Character Studies of Four Penicillium Fungi Selected From Varine Sediments

  • 摘要: 采用PCBs移除实验及其降解机理,分析研究从胶州湾海洋沉积物筛选的4株青霉真菌的好氧-厌氧降解的可能性,比较不同pH值、不同培养基条件对真菌生长的影响,获取真菌在好氧、厌氧条件下的生理学特性。结果表明,4株真菌更适合在海水MMM培养基上生长。pH值为6\sim 8时,均能正常生长;在偏碱性pH值下,更适合形成菌落。通过测定细胞数目建立的真菌生长曲线表明4株菌株均可在好氧、厌氧条件下形成菌落,在好氧条件下生长比厌氧条件下生长更好,这为进一步深入研究PCBs降解奠定了实验依据和技术基础。4株海洋真菌在不同母液的MMM培养基上的生长状况、其对不同pH值的适应能力以及在好氧一厌氧条件下的生长曲线均反映了菌株海洋来源的特性。

     

    Abstract: Based on our preliminary PCBs removing results, four dominant marine fungi, HTTD-Z0901, HTTD-Z0902, HTTD-Z0903 and HTTD-Z0904, were selected from Jiaozhou Bay sediments to study the growth characters under different culture mediums, pH values and aerobic, anaerobic conditions in order to ensure whether or not marine Penicillium fungi may undergo aerobic-anaerobic degradation. The results showed that the four marine fungi preferred MMM culture medium in which super pure water was replaced by sea water. And the four marine fungi all grew well at the pH value from 6 to 8, whereas alkaline condition was probably more appropriated. By biomass measurement, the four fungi could form colonies under aerobic, anaerobic conditions, and the growth of the four fungi in anaerobic condition were slower than those in aerobic condition. In conclusion, the growth characters of four fungi reflect the features of its original marine sources, and the four marine fungi may become a candidate in aerobic-anaerobic bioremediation.

     

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