Abstract: Based on the ambient noise data measured by Petrel underwater glider and sea surface wind speed reanalysis data in the southeastern Indian Ocean, the time-frequency characteristics of ambient noise from 4 to 20 kHz in this sea area are analyzed. It is verified that glider depth does not significantly affect the wind-related characteristics of ambient noise; the correlation coefficient between noise spectrum level and wind speed is quantitatively calculated; the linear function relationship between the noise spectrum level of each center frequency and the wind speed is obtained by least square fitting. The results show that: in the frequency band range less than 4 kHz, the correlation coefficient is below 0.2 due to the interference of the conductivity-temperature-depth (CTD) pump noise; in the frequency band greater than 4 kHz, the ambient noise spectrum level has a strong consistency with the variation trend of the sea surface wind speed, and the correlation coefficient is about 0.8 in 6-10 kHz. In the fequency range of 5-20 kHz, the fitting of noise spectrum level and wind speed have a linear function relationship, and as the center frequency increases, the average octaveband loss is about 5 dB; at 10 kHz, the fitting slope is the largest, the noise spectral levels are most sensitive to changes in sea surface wind speed.