Abstract:
To investigate the bacterial community structure, composition and diversity in surface seawater of Yongle atoll, Illumina MiSeq sequencing was used to analyze the difference in composition of the bacterial communities at six islands and reefs. The main environmental impact factors affecting bacteria community structure were explored and the functional genes of bacteria communty were also predicted. A total of 891 892 high-quality sequences clustering 7 499 optimized OTUs were obtained. These OTUs were subordinated to 49 phyla, 144 classes, 392 orders, 663 families, and 1 274 genera. The bacteria communities were dominated by
Proteobacteria,
Cyanobacteria,
Bacteroidetes,
Actinobacteria,
Patescibacteria and
Firmicutes. Redundancy analysis revealed that the conductivity, pH and temperature were the main environmental factors affecting the microbial assemblages. The results of PICRUSt analysis showed that 6 biological metabolic pathways and 46 subfunctionalities were predicted; a total of 399 functional genes at level 3 pathways were obtained, of which the most abundant functional genes were related to metabolic pathways and the biosynthesis of secondary metabolites. This study analyzes the bacterial community compositions in surface seawater and predicts their gene functions at six islands and reefs of Yongle atoll, providing a basic data for further investigation and development of microbial resources.